Organization of the Institute for Immunobiology and Human Genetics
1. Laboratory for Cellular Immunity
Field of
Investigation: cellular immunity, immunoregulation
Specific Field: ADA in cells, quantitative NBT.
Methods: phagocytosis, ADA activity.
Equipment: Haereus -80oC, Beckman Ultracentrifuge L-80, Nikon
Inverted Microscope.
Fields of Interest: innate and acquired immunity.
Person in Charge: Assist. Dr. Dejan Trajkov.
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2. Laboratory for Humoral Immunity
Field of
Investigation: immunoregulation, immunodiagnostics.
Specific Field: Acute phase proteins, immunoglobulin classes and
subclasses, tumor markers.
Methods: immunoturbidimetry, immunonephelometry, radial immunodiffusion,
luminometry, chromatography, immunoelectrophoresis.
Equipment: Behring TurbiTimer, Behring Nephelometer, Kodak luminometer,
Pharmacia chromatograph with phraction collector, spectrophotometer.
Fields of Interest: concentration of acute phase proteins, function of
antibodies, cancer diagnostics.
Person in Charge: Assist. Dr. Maja Jurhar-Pavlova.
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3. Laboratory for Allergology
Field of Investigation: Allergology.
Specific Field: Hypersensitivity.
Methods: Total and allergen specific IgE.
Equipment: UniCap 100.
Fields of Interest: investigation food, pollen, moulds, dender, home dust and other allergens.
Person in Charge:
Assist. Dr.
Aleksandar Petlichkovski.
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4. Laboratory for Molecular Biology and Human Genetics
Field of
Investigation: Tissue culture, mmunogenetics, transplantation, oncology, immunodeficiencies.
Specific Field: HLA-DNK typing, genetic variations in p53, ADA
izoenzymes.
Methods: isolation of DNA, PCR, horizontal electrophoresis, DNA
sequencing.
Equipment: Multiphor II, DNA 100 electrophoresis, horizontal
electrophoresis, UV transilluminator, Termocycler, ALFexpres II DNA sequencer.
Fields of Interest: HLA-DNA determination, genetic variations in
p53, ADA izoenzymes, immunodeficiencies.
Person in Charge: Prof. Dr. Mirko Spiroski
Oblast
na ispituvawe: kultura na
kletki i tkiva, imunogenetika, transplantacija, onkologija,
imunodeficiti.
Potesni/specifi~ni
podra~ja: funkcionalni
testovi na Li i drugi kletki, monoklonski protivtela, HLA-DNK tipizacija,
ADA izoenzimi.
Metodologii:
test za transformacija na Li, me{ana Li kultura, hibridizacija, izolacija
na DNK, PCR, horizontalna
elektroforeza, DNK sekvencionirawe.
Oprema:
Heraeus CO2
inkubator, horizontalna sterilna komora, centrifuga so ladewe, Termocycler,
ALFexpres II.
Dejnosti
koi bi bile od interes: reaktivnost
na kletki, apoptoza, proizvodstvo na monoklonski protivtela, opredeluvawe
HLA genotipizacija, polimorfizam na geni, genski mutacii, genetska varijabilnost
na p53, izoenzimi na ADA, imunodeficiti.
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Copyright
© 1998-2003; Institute of Immunobiology and Human Genetics,
Faculty of Medicine, Skopje, Republic of Macedonia
Most recent update: March 20, 2003
Author: Mirko
Spiroski, MD, PhD
Hosted by:
IIBHG, Skopje